Acrylamide, (500GM)

Acrylamide functions as a quencher of tryptophan fluorescence.[1] Acrylamide along with bis-acrylamide crosslinks to form polyacrylamide gels,[3] which is used to separate protein and other biomolecules. Bis-acrylamide is essential for crosslinking and acrylamide is required for the polymerization of monomers.[3] Acrylamide alkylates SH groups of cysteine and ε-NH2 lysine residues of proteins during electrophoresis.