Pseudomonas Isolation Agar Base, 500 G

Pseudomonas aeruginosa is an important human pathogen commonly found in nosocomial infections. It successfully combines adaptability to a variety of moist environments with a collection of potent virulence factors (1). Pseudomonas infections usually occur at any site where moisture tends to accumulate e. g. tracheostomies, in-dwelling catheters, burns, the external ear and weeping cutaneous wounds (2). Pseudomonas Isolation Agar Base, used for the selective isolation and identification of P. aeruginosa , is a modification of Medium A, originally formulated by King, Ward and Raney (3). The medium contains pigment-enhancing components and the selective agents, triclosan (4) which selectively inhibits non-pseudomonads. The pigment-enhancers i.e. potassium sulphate and magnesium chloride enhance the blue or blue-green pigment production by P. aeruginosa , thus aiding in its identification.

Peptic digest of animal tissue provides nitrogenous compounds and other essential growth nutrients. Glycerol is a source of energy and promotes pyocyanin i.e. pigment production which is characteristic of Pseudomonas (5, 6). Potassium sulphate and magnesium chloride enhance pyocyanin production. Triclosan (7) selectively inhibits gram-positive and gram-negative bacteria but Pseudomonas species are resistant to it. Some pyocyanin producing strains may also produce small amounts of fluorescein, resulting in the production of a blue-green to green pigment. Presumptive Pseudomonas should be further confirmed by performing biochemical tests, as some strains of Pseudomonas do not produce pyocyanin (8).

Directions: Suspend 45.03 grams in 1000 ml distilled water containing 20 ml glycerol. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Mix well and pour into sterile Petri plates.