ACP Acid Phosphatase Kit

The acid phosphatase assay protocol uses p-nitrophenyl phosphate (pNPP) as a phosphatase substrate which turns yellow (absorbance max 405 nm) when dephosphorylated by acid phosphatase.Acid phosphatase assay protocol summary: - add samples and standards to wells - add pNPP to samples - add acid phosphatase enzyme to standards - incubate for 60 min - add stop solution - analyze with a microplate readerNotes Acid phosphatases (AP) dephosphorylate phosphate groups from phosphate esters under acid conditions. Different acid phosphatase isozymes are found in different organs, and their serum levels are used as a diagnostic for disease in the corresponding organs. Elevated prostatic acid phosphatase levels may indicate the presence of prostate cancer and elevated tartrate-resistant acid phosphatase levels may indicate bone disease.