HEPES, =99.5% (titration) 250 G

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in vitro experiments on Mg.Application HEPES has been used:As a component in Danieau medium, which is used to prevent melanin pigment formation post gastrulation of zebra-fish embryo As a component of Hanks′ solution used in the degradation testing of Iron As a component of HEPES medium in the transfection of cell culture with plasmids Along with RPMI-1640, L-glutamine, FBS, Sodium pyruvate and β-mercaptoethanol for the culturing of rat insulinoma cells As a supplement in TCM199 medium for washing fresh oocytes required for nuclear transfer studies