Phenylmethylsulfonyl fluoride , 10 G

Synonyms: Phenylmethanesulfonyl fluoride; phenylmethylsulfonyl fluoride; α-Toluenesulfonyl fluoride; Benzylsulfonyl fluoride; PMSF; Phenylmethylsulfonyl fluorideEmpirical Formula (Hill Notation): C7H7FO2SFormula Weight: 174.19Linear Formula: C7H7FO2SMDL Number: MFCD00007424Purity: 98% (TLC)Storage: 20-25°CBeilstein Registry Number: 2088311UNSPSC Code: 12352200RIDADR: UN 2923 6.1(8) / PGIIApplication: PMSF (C7H7O2SF) inhibits serine proteases such as chymotrypsin, trypsin, thrombin, and the cysteine protease papain (reversible by DTT treatment). It does not inhibit metalloproteases, most cysteine proteases, or aspartic proteases. PMSF (phenylmethylsulfonyl fluoride) has been used for the preparation of protein extracts from tissues and cells prior to Western blotting.Biochem/physiol Actions: PMSF (phenylmethylsulfonyl fluoride) functions as a non-specific inhibitor of multiple proteases and other enzymes, such as palmityl coenzyme A deacylase, arylsulfatase A, acetylcholinesterase, trypsin and chymotrypsin. It is an irreversible inhibitor, and sulfonylates hydroxyl groups of the serine residues present in the active site of enzymes.