Investigator 24plex Kits , 100 Reactions

Product Details Investigator 24plex Kits are for human identification, allowing multiplex amplification of the CODIS (Combined DNA Index System) core loci, the ESS (European Standard Set) markers, plus SE33, DYS391 and Amelogenin. The kits include an innovative Quality Sensor, enabling invaluable additional data to be generated for quality control and performance tests. Furthermore, the Investigator 24plex GO! Kit enables direct amplification from reference samples without prior DNA extraction.Investigator 24plex Kits meet ISO 18385 requirements.Performance Investigator 24plex Kits use fast-cycling technology to provide reliable results in approximately 45–60 minutes.The Fast Reaction Mix 2.0 is based on proven QIAGEN multiplex PCR technology. It has been further optimized for ease of handling and increased speed and sensitivity with the Investigator 24plex QS Kit and for direct amplification without prior DNA extraction with the Investigator 24plex GO! Kit. Assay performance tested in the presence of increasing concentrations of inhibitors shows superior inhibitor resistance. This enables reliable, high-quality results from casework samples, but also from direct amplification of typical reference samples such as blood or epithelial cells on FTA paper.Investigator 24plex Kits were validated according to ENFSI and SWDAM guidelines.The recommended amount of DNA under standard conditions for the Investigator 24plex QS Kit is 0.5 ng. Internal validations demonstrated robust and balanced results with 0.2 to 2 ng DNA, and reliable results with 0.1 ng DNA.Principle Investigator 24plex Kits use QIAGEN's fast-cycling PCR technology for rapid and reliable co-amplification of the CODIS and ESS markers, plus SE33, DYS391 and Amelogenin. Carefully designed primers reduce overlap of loci alleles, thereby minimizing the likelihood of data misinterpretation.The innovative Quality Sensors provide additional information on sample quality and PCR success beyond the profile of the sample. With two Quality Sensors being amplified as a positive control, it is possible to determine:If amplification was successful If DNA was present in the sample If the sample was degraded If inhibitors in the sample prevented successful PCR This additional information will greatly enhance your data analysis and will help you to make crucial decisions with respect to further sample processing. The Quality Sensors will help you reduce unnecessary re-runs, saving you time and money.The two gender markers, Amelogenin and DYS391, have been optimized to produce small fragment sizes, making the gender typing extremely robust. Even for populations with Amelogenin-null mutations, this means no more failures with the essential gender typing, even with degraded or inhibited samples.